發(fā)布者：抗性基因網(wǎng) 時(shí)間：2023-06-12 瀏覽量：420
摘要
? ? ? 由于藥物在環(huán)境中的持續(xù)排放和處置，全球病原微生物群落中對某些藥物的耐藥性正在增加。這一現(xiàn)象導(dǎo)致幾個(gè)暴露的腸桿菌科物種產(chǎn)生了顯著的抗生素耐藥性，廢水處理廠和河流是重要的蓄水池。盡管抗生素耐藥性是一場悲劇，特別是在使用抗生素治療疾病方面，但表明嚴(yán)重程度、耐藥性物種和對這些病原體的分子水平了解的地方和地區(qū)研究很少，因此需要立即干預(yù)。因此，本研究通過表型和分子方法篩選耐藥病原體的基因，調(diào)查了污水處理池和接收水中是否存在耐藥病原體。在57個(gè)分析樣本中，發(fā)現(xiàn)18個(gè)（67%，n=27）克雷伯菌分離株、4個(gè)（80%，n=5）變形桿菌分離株、1個(gè)（100%，n=1）分離的銅綠假單胞菌和6個(gè)（18%，n=34）大腸桿菌對測試的抗生素中的至少1種具有耐藥性。大腸桿菌的多藥耐藥性（MDR）比例比克雷伯菌高83%，克雷伯桿菌為68.5%，銅綠假單胞菌分離株未顯示MDR。在20個(gè)具有抗生素抗性基因的細(xì)菌分離株中，大腸桿菌含有39%，其次是22%的克雷伯菌。這20個(gè)分離株中有11個(gè)（55%）含有磺酰胺類抗性基因：Sul 1（n=4）和Sul 2（n=7）。10個(gè)分離株（50%）含有四環(huán)素抗性基因，其中4個(gè)分離株顯示出Tet A，Tet B–1，5個(gè)分離株含有Tet D。在7個(gè)分離株中發(fā)現(xiàn)了β-內(nèi)酰胺酶（bla CTX-M和bla SHV）（35%）。這些抗生素耐藥性物種在城市接收和廢水中的存在，對現(xiàn)有藥物無法治愈的疾病傳播給人類和動(dòng)物構(gòu)成了威脅，危及公共衛(wèi)生安全。
Abstract
Antimicrobial resistance against certain medications in the pathogenic microbial community is globally increasing due to the continual discharge and disposal of pharmaceuticals in the environment. The phenomenon resulted in significant antibiotic resistance among several exposed Enterobacteriaceae species, with wastewater treatment plants (WWTPs) and rivers serving as significant reservoirs. Despite antibiotic resistance being a tragedy, particularly in treating diseases by using antibiotics, local and regional studies indicating the severity, resistant species and the molecular level insight into these pathogens are scarce, thus requiring immediate intervention. This study, therefore, investigated wastewater from wastewater treatment ponds and receiving waters for the presence of resistant pathogens through phenotypical and molecular approach screening of their genes. Among the 57 analyzed samples, 18 (67%, n = 27) isolates of Klebsiella spp.., 4 (80%, n = 5) isolates of Proteus spp., 1 (100%, n = 1) of isolated Pseudomonas aeruginosa and 6 (18%, n = 34) of E. coli found were resistant to at least 1 among the tested antibiotics. E. coli had an 83% higher proportion of multi-drug resistance (MDR) than Klebsiella spp.., which had 68.5%, and no MDR was shown by P. aeruginosa isolates. Among the 20 bacterial isolates of antibiotic-resistant genes, showed that E. coli harboured 39%, followed by 22% of Klebsiella spp. Eleven (11) isolates of these 20 (55%) contained sulphonamides resistant genes: Sul 1 (n = 4) and Sul 2 (n = 7). Ten (10) isolates (50%) contained the tetracycline-resistant genes in which 4 isolates showed the Tet A, Tet B – 1 and 5 isolates contained Tet D. β-lactamases (bla CTX-M and bla SHV) were found in 7 isolates (35%). The existence of these antibiotic-resistant species in the urban receiving and wastewater presents a threat of transmission of diseases to humans and animals that are not cured by the existing medications, jeopardizing public health safety.
https://www.sciencedirect.com/science/article/pii/S2590182622000145