發(fā)布者：抗性基因網(wǎng) 時(shí)間：2023-06-12 瀏覽量：445
摘要：
? ? ? 按照規(guī)定的程序采集了三個(gè)水體的水生生物樣本。使用曙紅亞甲基藍(lán)瓊脂（EMB）、甘露醇鹽瓊脂（MSA）、檸檬酸硫代硫酸鹽膽汁鹽蔗糖瓊脂（TCBS）、Muller Hinton瓊脂（MH）分離細(xì)菌。對(duì)分離株進(jìn)行了菌落、形態(tài)和生化鑒定。用血瓊脂對(duì)分離株進(jìn)行致病性試驗(yàn)，測(cè)定其溶血活性。采用Kirby-Bauer紙片擴(kuò)散法對(duì)標(biāo)準(zhǔn)抗生素的耐藥性狀況進(jìn)行了調(diào)查，并測(cè)定了分離株的多種抗生素耐藥性指標(biāo)。確定質(zhì)粒圖譜，并使用分子手段鑒定抗性基因。分離到的細(xì)菌種類為韋氏菌、東方弧菌和黃微球菌。Veillonella Spp顯示β溶血，而Microccus luteus顯示α溶血，Vibrio orientalis顯示γ溶血?？股孛舾行詼y(cè)試顯示，所有分離株都有不同程度的抗生素耐藥性，并且根據(jù)其耐藥性水平，有多種抗生素耐藥性指數(shù)（MARi）。韋略內(nèi)拉。spp.具有0.3的MARi，而M.luteus和V.orietalis具有0.5的MARi。質(zhì)粒圖譜顯示所有分離株都具有帶寬為10000bp的質(zhì)粒。β-內(nèi)酰胺酶基因（bla SHV）陽性。只有弧菌對(duì)氨基糖苷類（aac（3）-IV）的耐藥性呈陽性。東方弧菌（Vibrio orientalis）和黃微球菌（Microccus luteus）具有對(duì)喹諾酮類藥物的耐藥性基因（qnrA基因），而所有分離株都具有對(duì)所用磺酰胺類藥物的抗性基因（sul1基因）。這項(xiàng)工作的結(jié)果確定了從水生棲息地分離的細(xì)菌的抗生素耐藥性狀況，并表明它們?cè)诳股啬退幮訟MR和抗生素耐藥性基因（ARGs）傳播中的重要作用
Abstract:?
Aquatic samples from three water bodies were collected following laid down procedures. The bacteria were isolated using, Eosin Methylene Blue agar (EMB), Mannitol salt agar (MSA), Thiosulfate-Citrate-Bile Salts-Sucrose (TCBS) Agar, Muller Hinton (MH). The isolates were subjected to colonial, morphological and biochemical characterization. Pathogenicity test was carried out on the isolates using blood agar to determine their hemolytic activities. Antibiotic resistance status was also investigated against standard antibiotics using the Kirby Bauer disk diffusion method and the multiple antibiotic resistance indices of the isolates were also determined. The plasmid profiles were determined and the resistance genes were identified using molecular means. The bacteria species isolated were Veillonella Spp, Vibrio orientalis and Micrococcus luteus. Veillonella Spp showed Beta hemolysis while Micrococcus luteus showed alpha hemolysis and Vibrio orientalis showed gamma hemolysis. Antibiotic susceptibility test showed all isolates to be antibiotic resistant to varying degrees and multiple antibiotic resistance indices (MARi) depending on their level of resistance. Veillonella. spp. had MARi of 0.3 while M. luteus and V. orietalis had MARi of 0.5. Plasmid profiling showed all isolates to have plasmids with bandwidth of 10,000 bp. Vibrio spp and Veillonella Spp were positive for beta lactamase genes (bla SHV). Only Vibrio was positive for resistance to aminoglycosides (aac(3)-IV). Vibrio orientalis and Micrococcus luteus were found to have the resistance genes for quinolones (qnrA gene) while all the isolates possess genes against the sulphonamides (sul1 gene) used. The result of this work establishes the antibiotic resistance status of bacteria isolated from aquatic habitat and suggests their important role in the spread of antimicrobial resistance AMR, and antibiotic resistance genes (ARGs)
https://gsconlinepress.com/journals/gscbps/content/resistance-genes-and-plasmid-profile-bacteria-isolated-selected-water-bodies-owo-metropolis