發(fā)布者:抗性基因網(wǎng) 時間:2023-06-12 瀏覽量:417
摘要
? ? ? 沙門氏菌是食源性感染的主要原因之一,可引起細(xì)菌性食源性疾病。我們調(diào)查了2013年至2018年間從中國貴州臨床標(biāo)本中采集的人類沙門氏菌分離株的血清型分布、多藥耐藥性(MDR)和β-內(nèi)酰胺酶耐藥性基因。從17家監(jiān)測醫(yī)院的臨床標(biāo)本中共收集到363株沙門氏菌分離株?;瑒幽囼炶b定出24種血清型。腸炎沙門氏菌(33.9%)、沙門氏菌4、[5]、12:i:-(24.0%)、鼠傷寒沙門氏菌、倫敦沙門氏菌和德比沙門氏菌為前五大血清型。2018年,最常見的血清型從腸炎沙門氏菌變?yōu)槭髠抽T氏菌。在363個沙門氏菌分離株中,97.5%的分離株對至少一類抗菌劑具有耐藥性。對于頭孢菌素類藥物,頭孢曲松的耐藥率最高,為10.5%,頭孢吡肟和頭孢西丁的耐藥率分別為8.0%和2.2%。301株(82.9%)沙門氏菌表現(xiàn)出耐多藥耐藥性。沙門氏菌4、[5]、12:i:-的耐多藥耐藥率最高,為94.2%,其次是倫敦沙門氏菌(91.3%)和鼠傷寒沙門氏桿菌(88.1%)。2013年至2017年,貴州沙門氏菌的多藥耐藥性從75.8%上升到86.7%。16株(4.4%)表現(xiàn)出廣泛耐藥性。共發(fā)現(xiàn)134種抗微生物耐藥性模式。241株(66.4%)分離株攜帶至少一種β-內(nèi)酰胺酶抗性基因。blaTEM基因(61.2%)是所有沙門氏菌分離株中最普遍的耐藥基因,其次是blaCTX-M基因(6.1%)和blaOXA-1基因(4.1%)。因此,應(yīng)進(jìn)一步加強(qiáng)對臨床患者耐多藥沙門氏菌分離株的系統(tǒng)和長期監(jiān)測。
Abstract
Salmonella, one of the major causes of foodborne infections, can cause bacterial foodborne illness. We investigated the serotype distribution, multidrug resistance (MDR), and β-lactamase resistance genes of human Salmonella isolates collected from clinical specimens in Guizhou, China, between 2013 and 2018. A total of 363 Salmonella isolates were collected from clinical specimens at 17 surveillance hospitals. Twenty-four serotypes were identified by sliding agglutination test. S. Enteritidis (33.9%), Salmonella 4,[5],12:i:- (24.0%), S. Typhimurium (16.3%), S. London (6.3%), and S. Derby (3.9%) were the top five serotypes. In 2018, the most common serotype changed from S. Enteritidis to S. Typhimurium. Among the 363 Salmonella isolates, 97.5% of isolates were resistant to at least one class of antimicrobial agents. For cephalosporins, ceftriaxone had the highest resistance rate of 10.5%, and cefepime and cefoxitin were 8.0% and 2.2%, respectively. Three hundred and one (82.9%) Salmonella isolates showed MDR. Salmonella 4,[5],12:i:- had the highest MDR rate with 94.2%, followed by S. London (91.3%) and S. Typhimurium (88.1%). Multidrug resistance rates of Salmonella isolates in Guizhou from 2013 to 2017 increased from 75.8% to 86.7%. Sixteen isolates (4.4%) showed extensive drug resistance. One hundred thirty-four antimicrobial resistance patterns were found. Two hundred and forty-one (66.4%) isolates carried at least one β-lactamase resistance gene. The blaTEM gene (61.2%) was the most prevalent resistant gene in all Salmonella isolates, followed by the blaCTX-M gene (6.1%) and blaOXA-1 gene (4.1%). Our findings showed that the MDR rate of Salmonella isolates from Guizhou province increased year by year. Therefore, systematic and long-term surveillance on MDR Salmonella isolates from clinical patients should be further strengthened.
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0282254